amylose resin (New England Biolabs)

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New England Biolabs amylose resin
Amylose Resin, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 7578 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/amylose+resin/Amylose+Resin/pmc13123352-7-0-3
Average 99 stars, based on 7578 article reviews

amylose resin - by Bioz Stars, 2026-09

99/100 stars

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Purification:

Article Title: Modular small RNA drives the emergence of virulence traits and environmental trade-offs in Vibrio cholerae.
Article Snippet: .. AR TI CL E IN P RE SS The phage MS2 coat protein fused to maltose-binding protein (MS2-MBP) was purified using Ni-affinity chromatography (HisPur Ni-NTA Purification Kit, Thermo Scientific) and immobilized on amylose resin (New England Biolabs), following manufacturer protocols. ..

Chromatography:

Incubation:

Article Title: High-throughput screening approach identifies substrate-selective Hsp104 variants that counter amyloid seeding with diminished off-target effects.
Article Snippet: .. The eluent was then added to amylose resin (NEB) and incubated for 90 min at 4◦C, washed with amylose wash buffer (50 mM HEPES, pH 7.4, 0.5 M NaCl, 10% glycerol, 2 mM DTT), and eluted with amylose elution buffer (50 mM HEPES, pH 7.4, 0.5 M NaCl, 10% glycerol, 2 mM DTT, 100 μM PMSF, 10 μM pepstatin, 10 mM maltose, and complete protease inhibitors). ..

Article Title: TCP21 negatively regulates photomorphogenesis by integrating phyB ‐ and HY5 ‐mediated signaling pathways in Arabidopsis
Article Snippet: .. Subsequently, 20 μl of pre‐equilibrated amylose resin (New England Biolabs) was added and incubated for an additional 2 h with gentle rotation. ..

Centrifugation:

Article Title: Biogenesis and downstream effects of 3′,5′ and 2′,3′ cAMP isomers in plants
Article Snippet: .. The cell lysate was clarified by centrifugation at 18,000 g for 35 min, and the supernatant was applied to amylose resin (NEB, catalog no. E8021L) or glutathione agarose resin (Thermo Fisher Scientific, catalog no. 16102BID). ..

Construct:

Article Title: A nucleotide-triggered molecular switch orchestrating septin polymerization
Article Snippet: .. Pulldown assays were performed using amylose resin (New England Biolabs) to assess complex formation between tagged and untagged septin constructs under defined nucleotide conditions. ..

Gentle:

Binding Assay:

Article Title: Systematic screening of archaeal MazF homologs reveals Tth-MazF1, a versatile, sequence-specific ribonuclease from Thermococcus thioreducens
Article Snippet: Supernatants were collected and filtered using a 0.8 μm syringe filter (Cytiva, 4618). .. Amylose resin (NEB, E8021) was packed into a 10 ml disposable gravity column (Bio-Rad) and equilibrated with 15 ml of column binding buffer (CB) containing protease inhibitors. ..

Review